diff --git a/packages/devextreme/js/__internal/ui/__tests__/__mock__/model/popup.ts b/packages/devextreme/js/__internal/ui/__tests__/__mock__/model/popup.ts
index bf04cf870ed7..5258919e6089 100644
--- a/packages/devextreme/js/__internal/ui/__tests__/__mock__/model/popup.ts
+++ b/packages/devextreme/js/__internal/ui/__tests__/__mock__/model/popup.ts
@@ -5,6 +5,9 @@ const CLASSES = {
   popupWrapper: 'dx-popup-wrapper',
   popupTitle: 'dx-popup-title',
   overlayContent: 'dx-overlay-content',
+  closeButton: 'dx-closebutton',
+  toolbar: 'dx-toolbar',
+  button: 'dx-button',
 };
 
 const SELECTORS = {
@@ -37,6 +40,15 @@ export class PopupModel {
     return this.getOverlayContent()?.getAttribute('role') ?? null;
   }
 
+  public getCloseButton(): HTMLElement | null {
+    return this.getOverlayContent()?.querySelector(`.${CLASSES.closeButton}`) ?? null;
+  }
+
+  public getToolbarButtons(): HTMLElement[] {
+    const buttons = this.getOverlayContent()?.querySelectorAll<HTMLElement>(`.${CLASSES.toolbar} .${CLASSES.button}`);
+    return Array.from(buttons ?? []);
+  }
+
   public isVisible(): boolean {
     return !!this.getOverlayContent();
   }
diff --git a/packages/devextreme/js/__internal/ui/__tests__/tooltip.aria_role.test.ts b/packages/devextreme/js/__internal/ui/__tests__/tooltip.aria_role.test.ts
index 793e598f0dce..bbdcc6676269 100644
--- a/packages/devextreme/js/__internal/ui/__tests__/tooltip.aria_role.test.ts
+++ b/packages/devextreme/js/__internal/ui/__tests__/tooltip.aria_role.test.ts
@@ -1,5 +1,5 @@
 import {
-  afterEach, beforeAll, describe, expect, it,
+  afterAll, afterEach, beforeAll, describe, expect, it,
 } from '@jest/globals';
 import fx from '@js/common/core/animation/fx';
 import $ from '@js/core/renderer';
@@ -56,4 +56,27 @@ describe('Tooltip overlay content aria role', () => {
       expect(model.getRole()).toBe(role);
     });
   });
+
+  describe('on a shown tooltip with focusable toolbar elements', () => {
+    const originalGetClientRects = Element.prototype.getClientRects;
+
+    beforeAll(() => {
+      // jsdom performs no layout, so without this stub nothing counts as visible
+      // and the toolbar buttons are never tabbable.
+      Element.prototype.getClientRects = () => [{}] as unknown as DOMRectList;
+    });
+
+    afterAll(() => {
+      Element.prototype.getClientRects = originalGetClientRects;
+    });
+
+    it('sets the role on the overlay content, not on the toolbar button, when toolbar items with a button are added', async () => {
+      const model = await createTooltip({});
+
+      model.getInstance().option('toolbarItems', [{ widget: 'dxButton', options: { text: 'OK' } }]);
+
+      expect(model.getRole()).toBe('dialog');
+      expect(model.getToolbarButtons().map((button) => button.getAttribute('role'))).toEqual(['button']);
+    });
+  });
 });
diff --git a/packages/devextreme/js/__internal/ui/popover/__tests__/popover.aria_role.test.ts b/packages/devextreme/js/__internal/ui/popover/__tests__/popover.aria_role.test.ts
index dc11ae95e127..812bf605baf2 100644
--- a/packages/devextreme/js/__internal/ui/popover/__tests__/popover.aria_role.test.ts
+++ b/packages/devextreme/js/__internal/ui/popover/__tests__/popover.aria_role.test.ts
@@ -1,5 +1,5 @@
 import {
-  afterEach, beforeAll, describe, expect, it,
+  afterAll, afterEach, beforeAll, describe, expect, it,
 } from '@jest/globals';
 import fx from '@js/common/core/animation/fx';
 import $ from '@js/core/renderer';
@@ -86,4 +86,72 @@ describe('Popover overlay content aria role', () => {
       expect(model.getRole()).toBe('tooltip');
     });
   });
+
+  describe('on a shown popover with focusable toolbar elements', () => {
+    const originalGetClientRects = Element.prototype.getClientRects;
+
+    beforeAll(() => {
+      // jsdom performs no layout, so without this stub nothing counts as visible
+      // and the toolbar buttons are never tabbable.
+      Element.prototype.getClientRects = () => [{}] as unknown as DOMRectList;
+    });
+
+    afterAll(() => {
+      Element.prototype.getClientRects = originalGetClientRects;
+    });
+
+    it('sets the role on the overlay content, not on the close button, when a title and a close button are enabled', async () => {
+      const model = await createPopover({});
+
+      model.getInstance().option({ showTitle: true, title: 'Title', showCloseButton: true });
+
+      expect(model.getRole()).toBe('dialog');
+      expect(model.getCloseButton()?.getAttribute('role')).toBe('button');
+    });
+
+    it('switches back to "tooltip" when the close button is hidden again', async () => {
+      const model = await createPopover({});
+      model.getInstance().option({ showTitle: true, title: 'Title', showCloseButton: true });
+
+      model.getInstance().option('showCloseButton', false);
+
+      expect(model.getRole()).toBe('tooltip');
+      expect(model.getCloseButton()).toBeNull();
+    });
+
+    it('sets the role on the overlay content, not on the toolbar button, when toolbar items with a button are added', async () => {
+      const model = await createPopover({});
+
+      model.getInstance().option('toolbarItems', [{ widget: 'dxButton', options: { text: 'OK' } }]);
+
+      expect(model.getRole()).toBe('dialog');
+      expect(model.getToolbarButtons().map((button) => button.getAttribute('role'))).toEqual(['button']);
+    });
+
+    it('applies a forced _popoverContentRole to the overlay content, not to the close button', async () => {
+      const model = await createPopover({ showTitle: true, title: 'Title', showCloseButton: true });
+
+      model.getInstance().option('_popoverContentRole', 'tooltip');
+
+      expect(model.getRole()).toBe('tooltip');
+      expect(model.getCloseButton()?.getAttribute('role')).toBe('button');
+
+      model.getInstance().option('_popoverContentRole', undefined);
+
+      expect(model.getRole()).toBe('dialog');
+      expect(model.getCloseButton()?.getAttribute('role')).toBe('button');
+    });
+
+    it('toggles aria-disabled on the overlay content when disabled changes', async () => {
+      const model = await createPopover({ showTitle: true, title: 'Title', showCloseButton: true });
+
+      model.getInstance().option('disabled', true);
+
+      expect(model.getOverlayContent().getAttribute('aria-disabled')).toBe('true');
+
+      model.getInstance().option('disabled', false);
+
+      expect(model.getOverlayContent().getAttribute('aria-disabled')).toBeNull();
+    });
+  });
 });
diff --git a/packages/devextreme/js/__internal/ui/popup/popup.ts b/packages/devextreme/js/__internal/ui/popup/popup.ts
index 94d52609f018..a1c78f6eb8e5 100644
--- a/packages/devextreme/js/__internal/ui/popup/popup.ts
+++ b/packages/devextreme/js/__internal/ui/popup/popup.ts
@@ -792,6 +792,10 @@ class Popup<
     });
   }
 
+  _getAriaTarget(): dxElementWrapper {
+    return this.$overlayContent();
+  }
+
   protected _getAriaRole(): string {
     return 'dialog';
   }
diff --git a/packages/devextreme/testing/tests/DevExpress.ui.widgets/popover.tests.js b/packages/devextreme/testing/tests/DevExpress.ui.widgets/popover.tests.js
index 276f63e40b2a..229fee29c9ed 100644
--- a/packages/devextreme/testing/tests/DevExpress.ui.widgets/popover.tests.js
+++ b/packages/devextreme/testing/tests/DevExpress.ui.widgets/popover.tests.js
@@ -20,6 +20,9 @@ const POPOVER_WITHOUT_TITLE_CLASS = 'dx-popover-without-title';
 const POPOVER_TITLE_CLASS = 'dx-popup-title';
 const POPUP_CONTENT_CLASS = 'dx-popup-content';
 const OVERLAY_CONTENT_CLASS = 'dx-overlay-content';
+const POPUP_CLOSE_BUTTON_CLASS = 'dx-closebutton';
+const TOOLBAR_CLASS = 'dx-toolbar';
+const BUTTON_CLASS = 'dx-button';
 
 const positionAtWindowCenter = function(element) {
     positionUtils.setup(element, {
@@ -3314,5 +3317,71 @@ QUnit.module('accessibility', {
             assert.strictEqual(instance.option('tabFocusLoopEnabled'), false, 'tabFocusLoopEnabled remains false due to _preventDialogContainerFocus');
         });
     });
+
+    QUnit.module('shown popover with focusable toolbar elements', () => {
+        const createShownPopover = (options) => new Popover($('#what'), $.extend({
+            target: '#where',
+            visible: true,
+            animation: null,
+        }, options));
+
+        const getCloseButton = (popover) => popover.$overlayContent().find(`.${POPUP_CLOSE_BUTTON_CLASS}`);
+
+        QUnit.test('enabling showTitle and showCloseButton at runtime should set the role on the overlay content and keep the close button role', function(assert) {
+            const popover = createShownPopover();
+
+            popover.option({ showTitle: true, title: 'Title', showCloseButton: true });
+
+            assert.strictEqual(popover.$overlayContent().attr('role'), 'dialog', 'overlay content role is dialog');
+            assert.strictEqual(getCloseButton(popover).attr('role'), 'button', 'close button keeps its role');
+        });
+
+        QUnit.test('hiding the close button at runtime should restore role="tooltip" on the overlay content', function(assert) {
+            const popover = createShownPopover({ showTitle: true, title: 'Title', showCloseButton: true });
+
+            popover.option('showCloseButton', false);
+
+            assert.strictEqual(popover.$overlayContent().attr('role'), 'tooltip', 'overlay content role is tooltip');
+            assert.strictEqual(getCloseButton(popover).length, 0, 'close button is removed');
+        });
+
+        QUnit.test('adding toolbar items with a button at runtime should set the role on the overlay content and keep the button role', function(assert) {
+            const popover = createShownPopover();
+
+            popover.option('toolbarItems', [{ widget: 'dxButton', options: { text: 'OK' } }]);
+
+            const $button = popover.$overlayContent().find(`.${TOOLBAR_CLASS} .${BUTTON_CLASS}`);
+
+            assert.strictEqual(popover.$overlayContent().attr('role'), 'dialog', 'overlay content role is dialog');
+            assert.strictEqual($button.length, 1, 'toolbar button is rendered');
+            assert.strictEqual($button.attr('role'), 'button', 'toolbar button keeps its role');
+        });
+
+        QUnit.test('runtime _popoverContentRole change should apply to the overlay content and keep the close button role', function(assert) {
+            const popover = createShownPopover({ showTitle: true, title: 'Title', showCloseButton: true });
+
+            popover.option('_popoverContentRole', 'tooltip');
+
+            assert.strictEqual(popover.$overlayContent().attr('role'), 'tooltip', 'forced role is applied to the overlay content');
+            assert.strictEqual(getCloseButton(popover).attr('role'), 'button', 'close button keeps its role');
+
+            popover.option('_popoverContentRole', undefined);
+
+            assert.strictEqual(popover.$overlayContent().attr('role'), 'dialog', 'computed role is restored on the overlay content');
+            assert.strictEqual(getCloseButton(popover).attr('role'), 'button', 'close button still keeps its role');
+        });
+
+        QUnit.test('runtime disabled change should toggle aria-disabled on the overlay content', function(assert) {
+            const popover = createShownPopover({ showTitle: true, title: 'Title', showCloseButton: true });
+
+            popover.option('disabled', true);
+
+            assert.strictEqual(popover.$overlayContent().attr('aria-disabled'), 'true', 'overlay content is marked disabled');
+
+            popover.option('disabled', false);
+
+            assert.strictEqual(popover.$overlayContent().attr('aria-disabled'), undefined, 'overlay content is not marked disabled');
+        });
+    });
 });
 
diff --git a/packages/devextreme/testing/tests/DevExpress.ui.widgets/tooltip.tests.js b/packages/devextreme/testing/tests/DevExpress.ui.widgets/tooltip.tests.js
index 338c6c17edca..4d20314c23d7 100644
--- a/packages/devextreme/testing/tests/DevExpress.ui.widgets/tooltip.tests.js
+++ b/packages/devextreme/testing/tests/DevExpress.ui.widgets/tooltip.tests.js
@@ -9,6 +9,8 @@ const TOOLTIP_CLASS = 'dx-tooltip';
 const TOOLTIP_WRAPPER_CLASS = 'dx-tooltip-wrapper';
 const DX_INVISIBILITY_CLASS = 'dx-state-invisible';
 const OVERLAY_CONTENT_CLASS = 'dx-overlay-content';
+const TOOLBAR_CLASS = 'dx-toolbar';
+const BUTTON_CLASS = 'dx-button';
 
 const wrapper = function() {
     return $('body').find('.' + TOOLTIP_WRAPPER_CLASS);
@@ -208,6 +210,22 @@ QUnit.module('accessibility', () => {
         assert.equal($overlayContent.attr('role'), 'dialog');
     });
 
+    QUnit.test('adding toolbar items with a button at runtime should set the role on the overlay content and keep the button role', function(assert) {
+        const tooltip = new Tooltip($('#tooltip'), {
+            target: '#target',
+            visible: true,
+            animation: null
+        });
+
+        tooltip.option('toolbarItems', [{ widget: 'dxButton', options: { text: 'OK' } }]);
+
+        const $overlayContent = tooltip.$overlayContent();
+        const $button = $overlayContent.find(`.${TOOLBAR_CLASS} .${BUTTON_CLASS}`);
+
+        assert.equal($overlayContent.attr('role'), 'dialog', 'overlay content role is dialog');
+        assert.equal($button.attr('role'), 'button', 'toolbar button keeps its role');
+    });
+
     QUnit.module('WCAG - dismissible', () => {
         QUnit.test('should hide visible tooltip on Escape key press', function(assert) {
             const tooltip = new Tooltip($('#tooltip'), {