diff --git a/dev-packages/e2e-tests/test-applications/aws-serverless-layer/tests/layer.test.ts b/dev-packages/e2e-tests/test-applications/aws-serverless-layer/tests/layer.test.ts
index 538b533967e0..d6134267858e 100644
--- a/dev-packages/e2e-tests/test-applications/aws-serverless-layer/tests/layer.test.ts
+++ b/dev-packages/e2e-tests/test-applications/aws-serverless-layer/tests/layer.test.ts
@@ -337,7 +337,9 @@ test.describe('Lambda layer', () => {
     const transactionEvent = await transactionEventPromise;
 
     expect(transactionEvent.contexts?.trace?.status).toBe('ok');
-    expect(transactionEvent.timestamp! - transactionEvent.start_timestamp!).toBeGreaterThanOrEqual(0.2);
+    // The span clock and the 200ms callback timer can disagree by a few ms. A span that ended when the
+    // handler returned would be near 0, so this margin still catches it.
+    expect(transactionEvent.timestamp! - transactionEvent.start_timestamp!).toBeGreaterThanOrEqual(0.15);
   });
 
   test('sets error status when a callback-style handler calls back with an error after it returned', async ({
@@ -360,7 +362,7 @@ test.describe('Lambda layer', () => {
     const [errorEvent, transactionEvent] = await Promise.all([errorEventPromise, transactionEventPromise]);
 
     expect(transactionEvent.contexts?.trace?.status).toBe('internal_error');
-    expect(transactionEvent.timestamp! - transactionEvent.start_timestamp!).toBeGreaterThanOrEqual(0.2);
+    expect(transactionEvent.timestamp! - transactionEvent.start_timestamp!).toBeGreaterThanOrEqual(0.15);
     expect(errorEvent.contexts?.trace?.trace_id).toBe(transactionEvent.contexts?.trace?.trace_id);
   });