diff --git a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
index b8e24abd1a00..02294e0278c4 100644
--- a/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
+++ b/packages/react-native/scripts/featureflags/ReactNativeFeatureFlags.config.js
@@ -981,17 +981,6 @@ const definitions: FeatureFlagDefinitions = {
       },
       ossReleaseStage: 'none',
     },
-    animatedShouldUseSingleOp: {
-      defaultValue: true,
-      metadata: {
-        dateAdded: '2024-02-05',
-        description:
-          'Enables an experimental mega-operation for Animated.js that replaces many calls to native with a single call into native, to reduce JSI/JNI traffic.',
-        expectedReleaseValue: true,
-        purpose: 'experimentation',
-      },
-      ossReleaseStage: 'none',
-    },
     deferFlatListFocusChangeRenderUpdate: {
       defaultValue: false,
       metadata: {
diff --git a/packages/react-native/src/private/animated/NativeAnimatedHelper.js b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
index 03d7f5c1c23b..da9fb941aebb 100644
--- a/packages/react-native/src/private/animated/NativeAnimatedHelper.js
+++ b/packages/react-native/src/private/animated/NativeAnimatedHelper.js
@@ -56,7 +56,6 @@ const singleOpQueue: Array<unknown> = [];
 const isSingleOpBatching =
   Platform.OS === 'android' &&
   NativeAnimatedModule?.queueAndExecuteBatchedOperations != null &&
-  ReactNativeFeatureFlags.animatedShouldUseSingleOp() &&
   !ReactNativeFeatureFlags.cxxNativeAnimatedEnabled();
 let flushQueueImmediate = null;
 
diff --git a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
index cebd1193a628..521776f66b79 100644
--- a/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
+++ b/packages/react-native/src/private/featureflags/ReactNativeFeatureFlags.js
@@ -4,7 +4,7 @@
  * This source code is licensed under the MIT license found in the
  * LICENSE file in the root directory of this source tree.
  *
- * @generated SignedSource<<5e80ae1ef75d1fbf51fd94cd5495157f>>
+ * @generated SignedSource<<7b617acfec15f418c1d9fca60fdb3dd5>>
  * @flow strict
  * @noformat
  */
@@ -32,7 +32,6 @@ export type ReactNativeFeatureFlagsJsOnly = Readonly<{
   animatedDeferStartOfTimingAnimations: Getter<boolean>,
   animatedForceNativeDriver: Getter<boolean>,
   animatedShouldSyncValueBeforeStartCallback: Getter<boolean>,
-  animatedShouldUseSingleOp: Getter<boolean>,
   deferFlatListFocusChangeRenderUpdate: Getter<boolean>,
   enableImperativeEvents: Getter<boolean>,
   enableNativeEventTargetEventDispatching: Getter<boolean>,
@@ -155,11 +154,6 @@ export const animatedForceNativeDriver: Getter<boolean> = createJavaScriptFlagGe
  */
 export const animatedShouldSyncValueBeforeStartCallback: Getter<boolean> = createJavaScriptFlagGetter('animatedShouldSyncValueBeforeStartCallback', true);
 
-/**
- * Enables an experimental mega-operation for Animated.js that replaces many calls to native with a single call into native, to reduce JSI/JNI traffic.
- */
-export const animatedShouldUseSingleOp: Getter<boolean> = createJavaScriptFlagGetter('animatedShouldUseSingleOp', true);
-
 /**
  * Use the deferred cell render update mechanism for focus change in FlatList.
  */